Regulatory

Part:BBa_M36802:Design

Designed by: Bradley Hammoor   Group: Stanford BIOE44 - S11   (2014-04-30)


alcR operator/promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The mechanism by which the complex binds and inhibits/activates this region is currently unknown.


Source

This concept comes from the 2011 iGEM ETH Zurich team. (http://2011.igem.org/Team:ETH_Zurich). It also comes from extensive research done by Beatrice Felenbok. The sequence is the highest affinity binding site for alcR-acetaldehyde complex. (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC364357/pdf/molcellb00027-0040.pdf)

References